Bruker Catalyst BioAFM Atomic Force Microscope

FOM Name:  AFM Bruker BioAFM
Model Number:  Bruker BioScope Catalyst
Contact: Julie Morasch (morasch@wisc.edu, 608-262-3613)
Center:  NIAC
Location:  MSE Room 177A

The Bruker BioScope Catalyst AFM is designed for use with biological materials and is positioned on an inverted microscope, allowing for the integration of AFM imaging with optical imaging. This arrangement allows for the imaging of larger samples, including samples on glass slides or in glass bottom dishes.  The Catalyst has both PeakForce Tapping and the Nanomechanics software package, which allows the user to measure elastic modulus values and modulus maps for a wide range of materials.

Catalyst Overview and Examples

This is an accordion element with a series of buttons that open and close related content panels.

Configuration

Axiovert 200 Microscope

X-Cite Mercury Arc Lamp with DAPI (blue), GFP (green) and CY3 (red) filters

Objectives:

1)    A-Plan 10x/0.25air, WD = 4.5

2)    EC Plan-Neofluar 10x/0.3 air, WD = 5.2

3)    C-Apochromat 10x/0.45 water, WD = 1.8

4)    Plan Apochromat 20x/0.8,WD = 0.55

5)    C-Apochromat 40x/1.2water, WD = 0.28

6)    Fluar 40x/1.3 oil, WD = 0.16

7)    C-Apochromat 63x/1.2 water, WD = 0.28

8)    Plan-Apochromat 63x/1.4 oil, WD = 0.19

 

AFM Specifications

X-Y Scan Range 150µm, open-loop or closed-loop operation

Z Scan Range 20µm, open-loop or closed-loop operation

Deflection Detection IR super luminescent diode (SLD ), 850nm

Height Noise

<0.1nm RMS (air);

<0.2nm RMS (fluid)

(typical with appropriate vibration isolation)

Force Noise Thermally-limited, PicoForce-quality force measurements, <10pN RMS for cantilever with k=20pN/nm

XY Sample Stage

Motorized stage with 10x10mm range; includes magnetic sample clamps for 1x3in. slides, 25mm coverslips, 35 and 60mm plastic petri dishes, and 50mm glass bottom petri dishes

 

AFM Accessories

 MIRO Software

Allows real-time import and registration of optical and AFM images; optical images can be used to guide AFM imaging or force measurements; offline features allow image overlay, adjustment of colors and opacities, and flexible data export options

Perfusion Cells

Petri dish perfusion for 50mm glass-bottom petri dishes; Microvolume perfusion cell (<60µ L cell volume); Perfusing Stage Incubator for long-duration live-cell research

Sample Heating

Enables operation up to 40°C in liquid; physiological temperature range imaging (up to 40°C)

Top-View Optics

Allows use with non-transparent samples while AFM remains mounted on the inverted microscope

Nanomechanics Package

PeakForce QNM, Quantitative Force-Volume Mapping; single force curves; and comprehensive suite of force curve analysis tools

 

Links:

Bruker website

Probe selection guide

Peak force QNM

Peak force QNM with biological materials