FOM Name: Confocal Andor Spinning Disk
Model: Andor CSU-X Yokogawa Spinning Disk Confocal
Contact: Julie Morasch (morasch@wisc.edu, 608-262-3613)
Center: NIAC
Location: ECB Room 2043E
The Andor Spinning Disk confocal is an optical imaging technique with increased resolution as compared to widefield microscopy. Higher scanning speeds are possible with spinning disk confocal, decreasing the potential for sample photobleaching and phototoxicity.
The confocal is positioned on a Nikon Ti-E inverted microscope with Zyla sCMOS camera and include both a FRAPPA laser and a micropoint laser for photobleaching and wounding experiments.
This is an accordion element with a series of buttons that open and close related content panels.
Configuration
Yokogawa CSU-X1 Spinning Disk Unit, 5000 rpm disk speed, 50 um pinhole
Zyla sCMOS camera
Nikon TI microscope
LED BF light source
ASI Piezo z stage
Andor FRAPPA head
Micropoint with N2 pulsed laser (337 nm)
Excitation Sources:
405, 488, 561, 640
Emission Filters:
447/60nm, 525/30, 607/36, 685/40, quad (440/40, 521/21, 607/34, 700/45)
Objectives:
1) Plan Apo 10x/0.45air, WD = 4.0
2) Plan Apo 20x/0.75, WD = 1.0
3) Plan Apo 60x/1.4 oil, WD = 0.18
